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second-generation high-throughput sequencing  (Illumina Inc)


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    Structured Review

    Illumina Inc second-generation high-throughput sequencing
    Second Generation High Throughput Sequencing, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second-generation+high-throughput+sequencing/high+throughput+next+generation+sequencing/pmc12225625-50-1-5
    Average 90 stars, based on 1 article reviews
    second-generation high-throughput sequencing - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement of second-generation high-throughput sequencing technologies, particularly Illumina, the utilization of sex markers based on SNP/indel variants has increased to approximately 51% [ , ].

    Article Title: The bacterial composition signatures of perianal abscess and origin of infecting microbes
    Article Snippet: Samples were subjected to second-generation high-throughput Illumina sequencing, and bacterial community compositions were obtained for each sample.

    Article Title: Bacterial Communities and Prediction of Microbial Metabolic Pathway in Rice Wine Koji From Different Regions in China
    Article Snippet: The second-generation high-throughput sequencing technology of Illumina MiSeq makes up for the shortcomings of the aforementioned technology, allowing the measurement of millions of DNA molecules at the same time and the analysis of the gene composition and functional characteristics of specific microbial populations based on their gene sequences.

    Article Title: Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil
    Article Snippet: The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics.

    Article Title: Genome Resequencing and Transcriptome Analysis Reveal the Genetic Diversity of Wolfiporia cocos Germplasm and Genes Related to High Yield.
    Article Snippet: Wolfiporia cocos is a saprophytic fungus belonging to the phylum Basidiomycota.. The dried sclerotium of this organism has been widely used in traditional Chinese medicine for several thousand years and it is prescribed in many formulations.. The W. cocos germplasm resources are complex and diverse, and the molecular mechanisms underlying the growth and development of its sclerotia are unclear.

    Article Title: Interactive effects between soil properties and bacterial communities in tomato rhizosphere under the application of microbial agents
    Article Snippet: Using second-generation high-throughput sequencing technology (Illumina, San Diego, CA, USA) to sequence the 16S rRNA gene of tomato soil microorganisms; According to E Z.N.A.

    Article Title: Assessment of ITS1, ITS2, 5′-ETS, and trnL-F DNA Barcodes for Metabarcoding of Poaceae Pollen
    Article Snippet: We aimed to design primers to amplify the 5′-ETS fragment up to 600 bp in length so it could be fully sequenced using second-generation high-throughput sequencing (2 × 300 bp maximum length for the Illumina paired-end sequencing).

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement 521 of second-generation high-throughput sequencing technologies, particularly Illumina, the 522 utilization of sex markers based on SNP/InDel variants has increased to approximately 51% 523 [16, 73-78].

    Sequencing:

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement of second-generation high-throughput sequencing technologies, particularly Illumina, the utilization of sex markers based on SNP/indel variants has increased to approximately 51% [ , ].

    Article Title: The bacterial composition signatures of perianal abscess and origin of infecting microbes
    Article Snippet: Samples were subjected to second-generation high-throughput Illumina sequencing, and bacterial community compositions were obtained for each sample.

    Article Title: Bacterial Communities and Prediction of Microbial Metabolic Pathway in Rice Wine Koji From Different Regions in China
    Article Snippet: The second-generation high-throughput sequencing technology of Illumina MiSeq makes up for the shortcomings of the aforementioned technology, allowing the measurement of millions of DNA molecules at the same time and the analysis of the gene composition and functional characteristics of specific microbial populations based on their gene sequences.

    Article Title: Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil
    Article Snippet: The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics.

    Article Title: Genome Resequencing and Transcriptome Analysis Reveal the Genetic Diversity of Wolfiporia cocos Germplasm and Genes Related to High Yield.
    Article Snippet: Wolfiporia cocos is a saprophytic fungus belonging to the phylum Basidiomycota.. The dried sclerotium of this organism has been widely used in traditional Chinese medicine for several thousand years and it is prescribed in many formulations.. The W. cocos germplasm resources are complex and diverse, and the molecular mechanisms underlying the growth and development of its sclerotia are unclear.

    Article Title: Interactive effects between soil properties and bacterial communities in tomato rhizosphere under the application of microbial agents
    Article Snippet: Using second-generation high-throughput sequencing technology (Illumina, San Diego, CA, USA) to sequence the 16S rRNA gene of tomato soil microorganisms; According to E Z.N.A.

    Article Title: Assessment of ITS1, ITS2, 5′-ETS, and trnL-F DNA Barcodes for Metabarcoding of Poaceae Pollen
    Article Snippet: We aimed to design primers to amplify the 5′-ETS fragment up to 600 bp in length so it could be fully sequenced using second-generation high-throughput sequencing (2 × 300 bp maximum length for the Illumina paired-end sequencing).

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement 521 of second-generation high-throughput sequencing technologies, particularly Illumina, the 522 utilization of sex markers based on SNP/InDel variants has increased to approximately 51% 523 [16, 73-78].

    Software:

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement of second-generation high-throughput sequencing technologies, particularly Illumina, the utilization of sex markers based on SNP/indel variants has increased to approximately 51% [ , ].

    Article Title: The bacterial composition signatures of perianal abscess and origin of infecting microbes
    Article Snippet: Samples were subjected to second-generation high-throughput Illumina sequencing, and bacterial community compositions were obtained for each sample.

    Article Title: Bacterial Communities and Prediction of Microbial Metabolic Pathway in Rice Wine Koji From Different Regions in China
    Article Snippet: The second-generation high-throughput sequencing technology of Illumina MiSeq makes up for the shortcomings of the aforementioned technology, allowing the measurement of millions of DNA molecules at the same time and the analysis of the gene composition and functional characteristics of specific microbial populations based on their gene sequences.

    Article Title: Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil
    Article Snippet: The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics.

    Article Title: Genome Resequencing and Transcriptome Analysis Reveal the Genetic Diversity of Wolfiporia cocos Germplasm and Genes Related to High Yield.
    Article Snippet: Wolfiporia cocos is a saprophytic fungus belonging to the phylum Basidiomycota.. The dried sclerotium of this organism has been widely used in traditional Chinese medicine for several thousand years and it is prescribed in many formulations.. The W. cocos germplasm resources are complex and diverse, and the molecular mechanisms underlying the growth and development of its sclerotia are unclear.

    Article Title: Interactive effects between soil properties and bacterial communities in tomato rhizosphere under the application of microbial agents
    Article Snippet: Using second-generation high-throughput sequencing technology (Illumina, San Diego, CA, USA) to sequence the 16S rRNA gene of tomato soil microorganisms; According to E Z.N.A.

    Article Title: Assessment of ITS1, ITS2, 5′-ETS, and trnL-F DNA Barcodes for Metabarcoding of Poaceae Pollen
    Article Snippet: We aimed to design primers to amplify the 5′-ETS fragment up to 600 bp in length so it could be fully sequenced using second-generation high-throughput sequencing (2 × 300 bp maximum length for the Illumina paired-end sequencing).

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement 521 of second-generation high-throughput sequencing technologies, particularly Illumina, the 522 utilization of sex markers based on SNP/InDel variants has increased to approximately 51% 523 [16, 73-78].

    Functional Assay:

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement of second-generation high-throughput sequencing technologies, particularly Illumina, the utilization of sex markers based on SNP/indel variants has increased to approximately 51% [ , ].

    Article Title: The bacterial composition signatures of perianal abscess and origin of infecting microbes
    Article Snippet: Samples were subjected to second-generation high-throughput Illumina sequencing, and bacterial community compositions were obtained for each sample.

    Article Title: Bacterial Communities and Prediction of Microbial Metabolic Pathway in Rice Wine Koji From Different Regions in China
    Article Snippet: The second-generation high-throughput sequencing technology of Illumina MiSeq makes up for the shortcomings of the aforementioned technology, allowing the measurement of millions of DNA molecules at the same time and the analysis of the gene composition and functional characteristics of specific microbial populations based on their gene sequences.

    Article Title: Impact of Different Biochars on Microbial Community Structure in the Rhizospheric Soil of Rice Grown in Albic Soil
    Article Snippet: The abundance, diversity index, and community structure of the soil bacterial 16SrRNA gene V3 + V4 region and the fungal internal transcribed spacer-1 (ITS1) region were analyzed using Illumina second-generation high-throughput sequencing technology on the MiSeq platform with related bioinformatics.

    Article Title: Genome Resequencing and Transcriptome Analysis Reveal the Genetic Diversity of Wolfiporia cocos Germplasm and Genes Related to High Yield.
    Article Snippet: Wolfiporia cocos is a saprophytic fungus belonging to the phylum Basidiomycota.. The dried sclerotium of this organism has been widely used in traditional Chinese medicine for several thousand years and it is prescribed in many formulations.. The W. cocos germplasm resources are complex and diverse, and the molecular mechanisms underlying the growth and development of its sclerotia are unclear.

    Article Title: Interactive effects between soil properties and bacterial communities in tomato rhizosphere under the application of microbial agents
    Article Snippet: Using second-generation high-throughput sequencing technology (Illumina, San Diego, CA, USA) to sequence the 16S rRNA gene of tomato soil microorganisms; According to E Z.N.A.

    Article Title: Assessment of ITS1, ITS2, 5′-ETS, and trnL-F DNA Barcodes for Metabarcoding of Poaceae Pollen
    Article Snippet: We aimed to design primers to amplify the 5′-ETS fragment up to 600 bp in length so it could be fully sequenced using second-generation high-throughput sequencing (2 × 300 bp maximum length for the Illumina paired-end sequencing).

    Article Title: Innovative approach for high-throughput exploiting sex-specific markers in Japanese parrotfish Oplegnathus fasciatus
    Article Snippet: Meanwhile, with the advancement 521 of second-generation high-throughput sequencing technologies, particularly Illumina, the 522 utilization of sex markers based on SNP/InDel variants has increased to approximately 51% 523 [16, 73-78].



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